Isolation and regeneration of protoplasts from leaf explants of Rhyncholaelia digbyana
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چکیده
منابع مشابه
Isolation and regeneration of protoplasts from Ectocarpus.
This article describes how to obtain isolated cells with no surrounding cell wall by enzymatic digestion of Ectocarpus filaments. The resultant protoplasts are totipotent and regenerate to produce individual algae under appropriate culture conditions. The yield of protoplasts and their capacity to regenerate are highly dependent on the Ectocarpus strain used, the stage of the life cycle, and th...
متن کاملEffective in vitro seed germination and direct regeneration from cotyledonary leaf explants of Nitraria schoberi
Nitraria schoberi (Ghar-e-Daghin in Persian) from Zygophyllaceae (Nitrariaceae) family is considered as a wonderful exquisite plant in terms of its economical, medicinal, animal feed, significant values and also its unique characteristics of being tolerant to the soil salinity, drought and erosion. On the other hand, this plant is challenging with severe problems such as seed dormancy, plant gr...
متن کاملLabeling and isolation of plasma membranes from corn leaf protoplasts.
A plasma membrane-enriched fraction has been isolated from corn leaf mesophyll protoplasts and its identity confirmed with the aid of an external label, diazotized [(125)I]iodosulfanilic acid. Gentle cell disruption enabled internal organelles to be maintained intact and thus facilitated separation from the plasma membrane. The plasma membrane-enriched fraction was devoid of chloroplast or mito...
متن کاملRegeneration from leaf protoplasts of Arabidopsis thaliana ecotype estland.
Protoplasts (2 x 10(7)/g fresh wt) were isolated from leaves of A. thaliana ecotype estland, with a viability of more than 90%. Protoplasts cultured in calcium alginate beads or layers showed division while culture in liquid or agarose beads failed to elicit any division. Effect of culture density showed highest frequency of division occurring at 5 x 10(5) while no division was seen when cultur...
متن کاملSHOOT REGENERATION FROM PROTOPLASTS OF PENNISETUM AMERICANUM L (MIL CEREAL)
A finely dispersed cell suspension was obtained from embryogenic calli of two genotypes of pearl millet (Pennisetum americanum L. Mil cereal). Protoplasts isolated from both cell suspensions divided and produced globular embryoids and calli. On an agar medium, the embryoids were induced to form albino shoots or albino plantlet- like structures
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ژورنال
عنوان ژورنال: African Journal of Biotechnology
سال: 2018
ISSN: 1684-5315
DOI: 10.5897/ajb2018.16540